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Dengue Virus Typing Nucleic Acid Test Kit (PCR-fluorescence probe method)
Selelekela
Setsi sena se reretsoe ho lemoha ho thaepa ka boleng ba "dengue virus nucleic acid" ho serum ea motho kapa lisampole tsa plasma.Sekhechana sena se ipapisitse le sekhechana se ikhethileng sa lefutso la mofuta oa 1 ~ 4 oa mofuta oa 1 ~ 4 ho rala li-primers tse khethehileng le li-fluorescent tsa TaqMan bakeng sa mofuta o mong le o mong, le ho lemoha ho lemoha le ho ngola ka potlako kokoana ea dengue ka PCR ea nako ea nnete ea fluorescent.
Mekhahlelo
* Mofuta oa mohlala: Serum kapa Plasma * Lisebelisoa tsa kopo: ABI 7500 Real-Time PCR System;Bio-Rad CFX96;Roche LightCycler480;Sistimi ea SLAN PCR. * Polokelo -25 ℃ ho 8 ℃ e sa buloa 'me e sirelelitsoe ho tloha khanyeng ea likhoeli tse 18Likaroloana 48T/Kit Metsoako e ka Sehloohong DENV-Mofuta oa karabelo motsoako, lyophilized 2 li-tubes Li-primers, probes, PCR reaction buffer, dNTPs, Enzyme, joalo-joalo. DENV taolo e ntle, e nang le lyophilized 1 tube Li-Plasmids tsa tandem dengue virus ea mofuta oa 1-4 likhechana tse shebiloeng Taolo e mpe (Metsi a hlwekisitsweng) 1.5mL Metsi a hloekisitsoeng Bukana ea Mosebelisi 1 yuniti / Tshebetso
•Ka potlako: Nako e khuts'oane ea ntlafatso ea PCR har'a sehlahisoa se tšoanang.•Kutloelo-bohloko e phahameng le bokhabane: E khothaletsa tlhahlobo ea pele bakeng sa kalafo ea kapele.•Bokhoni bo felletseng ba ho thibela tšitiso.Mehato ea ts'ebetso
